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KMID : 1161420100130020406
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Journal of Medicinal Food
2010 Volume.13 No. 2 p.406 ~ p.414
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Ethanolic Extracts of Brassica campestris spp. rapa Roots Prevent High-Fat Diet-Induced Obesity via β3-Adrenergic Regulation of White Adipocyte Lipolytic Activity
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An So-Jin
Han Jang-Il
Kim Min-Jung
Park Ji-Seon
Han Jong-Min
Baek Nam-In
Chung Hae-Gon
Choi Myung-Sook
Lee Kyung-Tae
Jeong Tae-Sook
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Abstract
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The influence of ethanolic extracts of Brassica campestris spp. rapa roots (EBR) on obesity was examined in imprinting control region (ICR) mice fed a high-fat diet (HFD) and in 3T3-L1 adipocytes. The ICR mice used were divided into regular diet, HFD, EBR (50?mg/kg/day EBR administered orally), and orlistat (10?mg/kg/day orlistat administered orally) groups. The molecular mechanism of the anti-obesity effect of EBR was investigated in 3T3-L1 adipocytes as well as in HFD-fed ICR mice. In the obese mouse model, both weight gain and epididymal fat accumulation were highly suppressed by the daily oral administration of 50?mg/kg EBR for 8 weeks, whereas the overall amount of food intake was not affected. EBR treatment induced the expression in white adipocytes of lipolysis-related genes, including β3-adrenergic receptor (β3-AR), hormone-sensitive lipase (HSL), adipose triglyceride lipase, and uncoupling protein 2. Furthermore, the activation of cyclic AMP-dependent protein kinase, HSL, and extracellular signal-regulated kinase was induced in EBR-treated 3T3-L1 cells. The lipolytic effect of EBR involved β3-AR modulation, as inferred from the inhibition by the β3-AR antagonist propranolol. These results suggest that EBR may have potential as a safe and effective anti-obesity agent via the inhibition of adipocyte lipid accumulation and the stimulation of β3-AR-dependent lipolysis.
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KEYWORD
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β3-adrenegic receptor, anti-obesity effect, Brassica campestris spp. rapa, lipolysis, white adipose tissue
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